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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
24/10/2023 |
Actualizado : |
24/10/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
NOVAS, R.; BASIKA, T.; WILLIAMSON, M. E.; FRESIA, P.; MENCHACA, A.; SCOTT, M. J. |
Afiliación : |
ROSSINA NOVAS, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; MEGAN E. WILLIAMSON, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAXWELL J. SCOTT, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. |
Título : |
Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Insect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS. |
ISSN : |
0962-1075 (print); 1365-2583 (electronic). |
DOI : |
10.1111/imb.12875 |
Idioma : |
Inglés |
Notas : |
Article history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- |
Contenido : |
The New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. |
Palabras claves : |
CRISPR/Cas9; Genome editing; Partnership for the goals - Goal 17; Pest control; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Screwworm; Sustainable Development Goals (SDGs); U6 promoter. |
Asunto categoría : |
L01 Ganadería |
URL : |
https://resjournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/imb.12875
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Marc : |
LEADER 02928naa a2200313 a 4500 001 1064356 005 2023-10-24 008 2023 bl uuuu u00u1 u #d 022 $a0962-1075 (print); 1365-2583 (electronic). 024 7 $a10.1111/imb.12875$2DOI 100 1 $aNOVAS, R. 245 $aIdentification and functional analysis of Cochliomyia hominivorax U6 gene promoters.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- 520 $aThe New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. 653 $aCRISPR/Cas9 653 $aGenome editing 653 $aPartnership for the goals - Goal 17 653 $aPest control 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aScrewworm 653 $aSustainable Development Goals (SDGs) 653 $aU6 promoter 700 1 $aBASIKA, T. 700 1 $aWILLIAMSON, M. E. 700 1 $aFRESIA, P. 700 1 $aMENCHACA, A. 700 1 $aSCOTT, M. J. 773 $tInsect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
Fecha actual : |
21/02/2014 |
Actualizado : |
08/02/2018 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
BAETHGEN, W.E. |
Afiliación : |
WALTER E. BAETHGEN, IFDC (Internacional Fertilizer Development Center). |
Título : |
Dinamica del nitrogeno en sistemas de rotación cultivos-pasturas: resumen |
Fecha de publicación : |
1991 |
Fuente / Imprenta : |
In: ASOCIACIÓN DE INGENIEROS AGRÓNOMOS REGIONALES; INIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA); SIMPOSIO SUSTENTABILIDAD DE LAS ROTACIONES CULTIVO - PASTURA, 8-9 AGOSTO 1991, PAYSANDÚ, URUGUAY. Resumenes. Montevideo (Uruguay): INIA, 1991. |
Páginas : |
p.1 |
Serie : |
(INIA Serie técnica; 11) |
ISSN : |
1688-9266 |
Idioma : |
Español |
Notas : |
Trabajos publicados en la Revista INIA Investigaciones Agronómicas 1992 v.1(1) |
Thesagro : |
ABONOS NITROGENADOS; DETERIORO DEL SUELO; FERTILIDAD DEL SUELO; NITROGENO; PRADERA TEMPORAL; ROTACION DE CULTIVOS; SOSTENIBILIDAD. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/8272/1/111219220807114352-p.1.pdf
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Marc : |
LEADER 00956naa a2200241 a 4500 001 1005913 005 2018-02-08 008 1991 bl uuuu u00u1 u #d 022 $a1688-9266 100 1 $aBAETHGEN, W.E. 245 $aDinamica del nitrogeno en sistemas de rotación cultivos-pasturas$bresumen 260 $c1991 300 $ap.1 490 $a(INIA Serie técnica; 11) 500 $aTrabajos publicados en la Revista INIA Investigaciones Agronómicas 1992 v.1(1) 650 $aABONOS NITROGENADOS 650 $aDETERIORO DEL SUELO 650 $aFERTILIDAD DEL SUELO 650 $aNITROGENO 650 $aPRADERA TEMPORAL 650 $aROTACION DE CULTIVOS 650 $aSOSTENIBILIDAD 773 $tIn: ASOCIACIÓN DE INGENIEROS AGRÓNOMOS REGIONALES; INIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA); SIMPOSIO SUSTENTABILIDAD DE LAS ROTACIONES CULTIVO - PASTURA, 8-9 AGOSTO 1991, PAYSANDÚ, URUGUAY. Resumenes. Montevideo (Uruguay): INIA, 1991.
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